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2021 Articles

Accepted articles

Medication control of flunixin in racing horses: possible detection times using Monte Carlo simulations.
Kuroda T, Minamijima Y, Nomura M, Yamashita S, Yamada M, Nagata S, Mita H, Tamura N, Fukuda K, Kuwano A, Kusano K, Toutain PL, Sato F.
Equine Vet. J., DOI 10.1111/evj.13532.

Screening for gene doping transgenes in horses via the use of massively parallel sequencing.
Maniego J, Pesko B, Habershon-Butcher J, Huggett J, Taylor P, Scarth J, Ryder E.
Gene Ther., DOI 10.1038/s41434-021-00279-1.

Establishment of a post-race biomarkers database and application of pathway analysis to identify potential biomarkers in post-race equine plasma.
Ohnuma K, Uchida T, Leung GN, Ueda T, Obara T, Ishii H.
Drug Test. Anal., DOI 10.1002/dta.3041.

Published articles

Plasma levels of a methadone constant rate infusion and their corresponding effects on thermal and mechanical nociceptive thresholds in dogs.
Amon T, Kästner SBR, Kietzmann M, Tünsmeyer J.
BMC Vet. Res., 2021, 17, 35.

The effect of serial administration of bicarbonate on plasma total CO2 concentrations in horses.
Bailey SR, Forbes G, Selvadurai N, McLarney K, Jones S, Steel CM.
Drug Test. Anal., 2021, 13, 397–403.

A quick approach for medetomidine enantiomers determination in dog plasma by chiral LC-MS/MS and application to a pharmacokinetic study.
Bardhi A, Zaghini A, Levionnois O, Barbarossa A.
Drug Test. Anal., 2021, 13, 1249–1255.

Investigation of equine in vivo and in vitro derived metabolites of the selective androgen receptor modulator (SARM) ACP-105 for improved doping control.
Broberg MN, Knych H, Bondesson U, Pettersson C, Stanley S, Thevis M, Hedeland M.
Metabolites, 2021, 11, 85.

Quantification of 17 endogenous and exogenous steroidal hormones in equine and bovine blood for doping control with UHPLC-MS/MS.
Caprioli G, Genangeli M, Mustafa AM, Petrelli R, Ricciutelli M, Sagratini G, Sartori S, Laus F, Vittori S, Cortese M.
Pharmaceuticals (Basel), 2021, 14, 393.

A duplex qPCR assay for human erythropoietin (EPO) transgene to control gene doping in horses.
Cheung HW, Wong KS, Lin VYC, Wan TSM, Ho ENM.
Drug Test. Anal., 2021, 13, 113–121.

Label-free proteomics for discovering biomarker candidates of RAD140 administration to castrated horses.
Cheung HW, Wong KS, To NS, Bond AJ, Farrington AF, Prabhu A, Curl P, Wan TSM, Ho ENM.
Drug Test. Anal., 2021, 13, 1034–1047.

Bioanalysis of niclosamide in plasma using liquid chromatography-tandem mass and application to pharmacokinetics in rats and dogs.
Choi HI, Kim T, Lee SW, Woo Kim J, Ju Noh Y, Kim GY, Jin Park H, Chae YJ, Lee KR, Kim SJ, Koo TS.
J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2021, 1179, 122862.

Equine metabolism of the selective androgen receptor modulator AC‐262536 in vitro and in urine, plasma and hair following oral administration.
Cutler C, Viljanto M, Taylor P, Habershon‐Butcher J, Muir T, Biddle S, Van Eenoo P.
Drug Test. Anal., 2021, 13, 369–385.

Transcriptomic markers of recombinant human erythropoietin micro-dosing in Thoroughbred horses.
Dahlgren AR, Knych HK, Arthur RM, Durbin-Johnson BP, Finno CJ.
Genes (Basel), 2021, 12, 1874.

Development of a standardized microflow LC gradient to enable sensitive and long-term detection of synthetic anabolic-androgenic steroids for high-throughput doping controls.
Delcourt V, Garcia P, Pottier I, Mansoibou N, Bache N, Glavieux Y, Chabot B, Perot I, André F, Loup B, Barnabé A, Popot MA, Bailly-Chouriberry L.
Anal. Chem., 2021, 93, 15590-15596.

Long-term detection of clodronate in equine plasma by liquid chromatography-tandem mass spectrometry.
Garcia P, Perot I, Loup B, Balssa F, Jaubert M, Delcourt V, Dujardin C, Popot MA, Bailly-Chouriberry L.
Drug Test. Anal., 2021, 13, 1527–1534.

Unravelling androgens in sport: Altrenogest shows strong activation of the androgen receptor in a mammalian cell bioassay.
Gillon A, Ho ENM, Chan GHM, Kauff A, Hughes G, Lund RA, Ashley Z, Wan TSM, Heather AK.
Drug Test. Anal., 2021, 13, 523–528.

Novel algorithms for comprehensive untargeted detection of doping agents in biological samples.
Guan F, You Y, Fay S, Li X, Robinson MA.
Anal. Chem., 2021, 93, 7746–7753.

Tolerance and pharmacokinetics of Galliprant™ administered orally to collies homozygous for MDR1-1Δ.
Heit MC, Mealey KL, King SB.
J. Vet. Pharmacol. Ther., 2021, 44, 705–713.

Pharmacokinetics of enflicoxib in dogs: Effects of prandial state and repeated administration.
Homedes J, Salichs M, Solà J, Menargues A, Cendrós JM, Encina G.
J. Vet. Pharmacol. Ther., 2021, 44, 888–901.

Stability of ketoprofen methylester in plasma of different species.
Hu SX, Ernst K, Benner CP, Feenstra KL.
Curr. Drug Metab., 2021, 22, 215–223.

Detection and longitudinal distribution of GW1516 and its metabolites in equine hair for doping control using liquid chromatography - high-resolution mass spectrometry.
Ishii H, Shibuya M, Leung GNW, Nozawa S, Yamashita S, Yamada M, Kushiro A, Kasashima Y, Okada J, Kawasaki K, Kijima-Suda I.
Rapid Commun. Mass Spectrom., 2021, 35, e9050.

Metabolic study of GW1516 in equine urine using liquid chromatography/electrospray ionization Q-Exactive high-resolution mass spectrometer for doping control.
Ishii H, Shibuya M, Leung GNW, Yamashita S, Yamada M, Kushiro A, Kasashima Y, Okada J, Kawasaki K, Kijima-Suda I.
Rapid Commun. Mass Spectrom., 2021, 35, e9028.

Metabolism and excretion of the benzodiazepine analog etizolam in the horse.
Johnson E, van Heemst J, Benavides J, Gray B.
Drug Test. Anal., 2021, 13, 583–594.

In vitro metabolism of slowly cleared GPR139 agonist TAK-041 using rat, dog, monkey and human hepatocyte models (HepatoPac): correlation with in vivo metabolism.
Kamel A, Bowlin S, Hosea N, Arkilo D, Laurenza A.
Drug Metab. Dispos., 2021, 49, 121–132.

Simultaneous analysis of 44 frequently abused corticosteroid drugs using polysaccharide-based chiral column-HRMS approach.
Karakka Kal AK, Karatt TK, Nalakath J, Subhahar MB, Koshy SA, Perwad Z, Muhammed Ajeebsanu MP.
Anal. Sci. Adv., 2021, 2, 427–439.

Towards an untargeted mass spectrometric approach for improved screening in equine anti-doping.
Keen B, Cawley A, Fouracre C, Pyke J, Fu S.
Drug Test. Anal., 2021, 13, 1001–1007.

Pharmacokinetic analysis of two different doses of simvastatin following oral administration in dogs.
Kim MS, Baek IH.
J. Vet. Pharmacol. Ther., 2021, 44, 333–341.

Determination of the route of excretion of robenacoxib (Onsior™) in cats and dogs: A pilot study.
King JN, Jung M.
J. Vet. Pharmacol. Ther., 2021, 44, 411–416.

Finding the proverbial needle: Non-targeted screening of synthetic opioids in equine plasma.
Klingberg J, Cawley A, Shimmon R, Fouracre C, Pasin D, Fu S.
Drug Test. Anal., 2021, 13, 977–989.

Single‐dose pharmacokinetics of mycophenolic acid following administration of immediate‐release mycophenolate mofetil in healthy beagle dogs.
Klotsman M, Sathyan G, Anderson WH.
J. Vet. Pharmacol. Ther., 2021, 44, 650–656.

Pharmacokinetics of transdermal flunixin meglumine and effects on biomarkers of inflammation in horses.
Knych HK, Arthur RM, Gretler SR, McKemie DS, Goldin S, Kass PH.
J. Vet. Pharmacol. Ther., 2021, 44, 745–753.

Pharmacokinetics and anti-inflammatory effects of flunixin meglumine as a sole agent and in combination with phenylbutazone in exercised Thoroughbred horses.
Knych HK, Arthur RM, McKemie DS, Baden RW, Seminoff K, Kass PH.
Equine Vet. J., 2021, 53, 102–116.

Identification and characterization of the enzymes responsible for the metabolism of the non‐steroidal anti‐inflammatory drugs, flunixin meglumine and phenylbutazone, in horses.
Knych HK, Finno CJ, Baden R, Arthur RM, McKemie DS.
J. Vet. Pharmacol. Ther., 2021, 44, 36–46.

Species difference in hydrolysis of an ester-type prodrug of levodopa in human and animal plasma: Different contributions of alpha-1 acid glycoprotein.
Kono K, Nunoya KI, Nakamura Y, Bi J, Mukunoki A, Takeo T, Nakagata N, Hitoshi M, Yamaura Y, Imawaka H, Watanabe H, Maruyama T.
Mol. Pharm., 2021, 18, 1985–1991.

Agomelatine: A novel melatonergic antidepressant. Method validation and first exploratory pharmacokinetic study in fasted and fed dogs.
Łebkowska-Wieruszewska B, DELLYZiółkowski H, Sartini I, Lisowski A, Kowalski CJ, Poapolathep A, Giorgi M.
Res. Vet. Sci., 2021, 139, 140–144.

Application of a non-target variable data independent workflow (vDIA) for the screening of prohibited substances in doping control testing.
Leung DKK, Wong ASY, Zhou QL, Wan TSM, Ho ENM.
Drug Test. Anal., 2021, 13, 1008–1033.

Quantitative determination of AZD3264, a selective Ikb-kinase IKK2 inhibitor, in dog plasma by solvent-induced phase transition extraction coupled with HPLC-MS/MS and its application to pharmacokinetic study in dogs.
Li G, Liang S, Qiao K, Wang C.
J. AOAC Int., 2021, 104, 348–354.

A sensitive HPLC-MS/MS method for the detection, resolution and quantitation of cathinone enantiomers in horse blood plasma and urine.
Loganathan D, Yi R, Patel B, Zhang J, Kong N.
Anal. Bioanal. Chem., 2021, 413, 2147–2161.

Identification of HIF stabilizer roxadustat and its possible metabolites in Thoroughbred horses for doping control.
Mathew B, Philip M, Perwad Z, Karatt TK, Caveney MR, Subhahar MB, Karakka Kal AK.
Drug Test. Anal., 2021, 13, 1203–1215.

Oral pharmacokinetics in beagle dogs of the mitragynine metabolite, 7-hydroxymitragynine.
Maxwell EA, King TI, Kamble SH, Raju KSR, Berthold EC, León F, Hampson A, McMahon LR, McCurdy CR, Sharma A.
Eur. J. Drug Metab. Pharmacokinet., 2021, 46, 459–463.

A validated method for the quantification of IOX-2, a potent prolyl hydroxylase inhibitor in equine urine and plasma using liquid chromatography-high-resolution mass spectrometry.
Mikhail E, Siccardi E, Bawazir A, Rajesh A, Prathyush S, Al Wazani DMK, Sabeek M, John T.
Drug Test. Anal., 2021, 13, 1178–1190.

Detection of methylphenidate in equine hair using liquid chromatography-high-resolution mass spectrometry.
Moeller BC, Flores L, Clifford A, Alarcio G, Mosburg M, Arthur RM.
Molecules, 2021, 26, 5798.

Evaluation of dried blood spots as an alternative sample matrix for equine anti-doping analysis.
Moeller BC, Yang Z.
Drug Test. Anal., 2021, 13, 386–396.

Doping detection in animals: A review of analytical methodologies published from 1990 to 2019.
Moreira F, Carmo H, de Pinho PG, de Lourdes Bastos M.
Drug Test. Anal., 2021, 13, 474–504.

Plasma and urine pharmacokinetics of two formulations of dexamethasone in greyhound dogs.
Morris T, Paine SW, Zahra P, Li E, Colgan S, Karamatic S.
J. Vet. Pharmacol. Ther., 2021, 44, 689–695.

Pharmacodynamics and plasma concentrations of dexmedetomidine with or without vatinoxan as a constant-rate infusion in horses anaesthetized with isoflurane—A pilot study.
Neudeck S, Twele L, Kopp V, Kästner S.
J. Vet. Pharmacol. Ther., 2021, 44, 754–765.

Pharmacokinetics of dipyrone in horses: A multi-dose, dose escalation study.
O'Banion MP, Sundman E, Edmonds M, Davis J.
J. Vet. Pharmacol. Ther., 2021, 44, 919–926.

Preliminary bioequivalence of an oral integrating film formulation containing meloxicam with a suspension formulation in beagle dogs.
Park JS, Kim S, Lee J, Choi JC, Kim Y, Park CW, Lee S.
J. Vet. Pharmacol. Ther., 2021, 44, 342–348.

Characterization of equine liver microsome-generated metabolites of growth hormone secretagogue small molecule ibutamoren.
Philip M, Karakka Kal AK, Subhahar MB, Perwad Z, Karatt TK.
Rapid Commun. Mass Spectrom., 2021, 35, e9201.

Metabolic studies of HIF stabilizers IOX2, IOX3, and IOX4 (in vitro) for doping control.
Philip M, Mathew B, Karatt TK, Perwad Z, Subhahar MB, Karakka Kal AK.
Drug Test. Anal., 2021, 13, 794–816.

Pharmacodynamics and pharmacokinetics of injectable pimobendan and its metabolite, O-desmethyl-pimobendan, in healthy dogs.
Pichayapaiboon P, Tantisuwat L, Boonpala P, Saengklub N, Boonyarattanasoonthorn T, Khemawoot P, Kijtawornrat A.
Front. Vet. Sci., 2021, 8, 656902.

The in vivo metabolism of Furazadrol in greyhounds.
Pranata A, Curtis B, Waller CC, Caldwell K, Zahra PW, Karamatic SL, McLeod MD.
Drug Test. Anal., 2021, 13, 1749–1757.

Multidose pharmacokinetics and safety of a modified, compounded theophylline product in dogs.
Reinhart JM, Perkowski C, Lester C, Campos V, Kadotani S, Li Z, McKiernan BC, Fries R.
J. Vet. Pharmacol. Ther., 2021, 44, 902–909.

Tiludronic acid can be detected in blood and urine samples from Thoroughbred racehorses over three years after last administration.
Riggs CM, Thompson SL, Wong JKY, So YM, Wan TSM, Robinson P, Stewart BD, Ho ENM.
Equine Vet. J., 2021, 53, 1287–1295.

The effect of posaconazole and isavuconazole on the pharmacokinetics of erdafitinib in beagle dogs by UPLC-MS/MS.
Ruan LH, Fan LL, Wang K, Zhang WQ, Wang XJ, Qiu XJ.
Front. Pharmacol., 2021, 12, 749169.

Pharmacokinetics and effects on arachidonic acid metabolism of low doses of cannabidiol following oral administration to horses.
Ryan D, McKemie DS, Kass PH, Puschner B, Knych HK.
Drug Test. Anal., 2021, 13, 1305–1317.

Pharmacokinetics of acetaminophen after intravenous and oral administration in fasted and fed Labrador retriever dogs.
Sartini I, Łebkowska-Wieruszewska B, Lisowski A, Poapolathep A, Cuniberti B, Giorgi M.
J. Vet. Pharmacol. Ther., 2021, 44, 28–35.

Kinetic disposition of diazepam and its metabolites after intravenous administration of diazepam in the horse: Relevance for doping control.
Schenk I, Machnik M, Broussou D, Meuly A, Roques BB, Lallemand E, Düe M, Röttgen H, Lagershausen H, Toutain PL, Thevis M.
J. Vet. Pharmacol. Ther., 2021, 44, 733–744.

Pharmacokinetics and pharmacodynamics of intravenous continuous rate infusion and repeated intramuscular administration of dexmedetomidine in standing horses.
Shane SE, Langston VC, Wills RW, Denney WS, Knych H, Fontenot RL, Meyer RE, Natalini CC.
J. Vet. Pharmacol. Ther., 2021, 44, 533–543.

Metabolic studies of selective androgen receptor modulators RAD140 and S-23 in horses.
So YM, Wong JKY, Choi TLS, Prabhu A, Stewart B, Farrington AF, Robinson P, Wan TSM, Ho ENM.
Drug Test. Anal., 2021, 13, 318–337.

Comparative in vitro metabolism of enflicoxib in dogs, rats, and humans: main metabolites and proposed metabolic pathways.
Solà J, Menargues À, Homedes J, Salichs M, Serafini MT, Encina G.
Drug Metab. Lett., 2021, 14, 206-218.

Systemic detectability of dexamethasone and prednisolone after eye drop application in horses.
Stucki M, Voegel CD, Binz TM, Kraemer T, Lavaud A, Voelter K.
Equine Vet. J., 2021, 53, 1250–1256.

Pyrotinib in vitro metabolite profiling via rat, dog and human hepatocytes by liquid chromatography-quadrupole/orbitrap mass spectrometry.
Su G, Qin L, Su X, Tao C.
Rapid Commun. Mass Spectrom., 2021, 35, e9195.

Detection and identification of ACP‐105 and its metabolites in equine urine using LC/MS/MS after oral administration.
Subhahar MB, Karakka Kal AK, Philip M, Karatt TK, Ibrahimwaseem N., Vazhat RA, Muhammed Ajeebsanu M.P.
Drug Test. Anal., 2021, 13, 299–317.

Simulated validation of intron-less transgene detection using DELLY for gene-doping control in horse sports.
Tozaki T, Ohnuma A, Kikuchi K, Ishige T, Kakoi H, Hirota K, Kusano K, Nagata SI.
Anim. Genet., 2021, 52, 759–761.

Robustness of digital PCR and real-time PCR against inhibitors in transgene detection for gene doping control in equestrian sports.
Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
Drug Test. Anal., 2021, 13, 1768–1775.

Design and storage stability of reference materials for microfluidic quantitative PCR-based equine gene doping tests.
Tozaki T, Ohnuma A, Kikuchi M, Ishige T, Kakoi H, Hirota KI, Kusano K, Nagata SI.
J. Equine Sci., 2021, 32, 125–134.

Comprehensive characterization of the PPAR-δ agonist GW501516 for horse doping control analysis.
Trevisiol S, Moulard Y, Delcourt V, Jaubert M, Boyer S, Tendon S, Haryouli H, Taleb W, Caroff M, Chabot B, Drif L, André F, Garcia P, Loup B, Barnabé A, Popot MA, Bailly-Chouriberry L.
Drug Test. Anal., 2021, 13, 1191–1202.

Pharmacokinetics and pharmacodynamics of intravenous and oral apixaban in horses.
Wallace GE, McKaba VF, Reinhart JM, Li Z, Austin S, Fries RC.
J. Vet. Pharmacol. Ther., 2021, 44, 724–732.

The pharmacokinetic effect of itraconazole and voriconazole on ripretinib in beagle dogs by UPLC-MS/MS technique.
Wang HJ, Zhou CY, Su YD, Gou KF, Geng XN, Qiu XJ.
Drug Des. Devel. Ther., 2021, 15, 4865–4873.

Development of an UPLC-MS/MS method for the quantitative analysis of upadacitinib in beagle dog plasma and pharmacokinetics study.
Wang MJ, Zhao YH, Fan C, Wang YJ, Wang XQ, Qiu XJ, Shen RL.
Drug Des. Devel. Ther., 2021, 15, 4167–4175.

Concentration profiles and safety of topically applied betulinic acid and NVX‐207 in eight healthy horses—A randomized, blinded, placebo‐controlled, crossover pilot study.
Weber LA, Puff C, Kalbitz J, Kietzmann M, Feige K, Bosse K, Rohn K, Cavalleri JMV.
J. Vet. Pharmacol. Ther., 2021, 44, 47–57.

Effect of sijunzi pills on pharmacokinetics of omeprazole in beagle dogs by HPLC-UV: A herb-drug interaction study.
Xia HL, Zhao XJ, Zhang YJ, Su XH, Sun B, Qiu XJ.
Evid. Based Complement. Alternat. Med., 2021, 4181196.

Quantitation of apremilast in beagle dogs plasma by UPLC-MS-MS and iIts application to pharmacokinetic studies.
Xiong W, Wang L, Zhang H, Tao X, Jiang X, Liu Z, Zhao J, Xu W.
J. Anal. Methods Chem., 2021, 8881076.

Simultaneous determination of naproxen and esomeprazole in beagle dog plasma by supercritical fluid chromatography-tandem mass spectrometry coupled with evaporation-free liquid-liquid extraction.
Yang Y, Li X, Zhou Q, Sun L, Zhang T.
J. Pharm. Biomed. Anal., 2021, 193, 113668.

Pharmacokinetic profiles of metamizole metabolites after intramuscular and intravenous administration in healthy Arabian horses.
Yılmaz İ, Maraş Z, Uğur Y, Özgür ME, Durmaz M, Ulusoy Hİ, Erdoğan S.
J. Vet. Pharmacol. Ther., 2021, 44, 927–936.

Use of high resolution/accurate mass full scan/data-dependent acquisition for targeted/non-targeted screening in equine doping control.
You Y, Proctor RM, Guo K, Li X, Xue E, Guan F, Robinson MA.
Anal. Methods, 2021, 13, 1565–l1575.

Absorption, metabolism and excretion of pictilisib, a potent pan-class I phosphatidylinositol-3-kinase (PI3K) inhibitor, in rats, dogs, and humans.
Yue Q, Khojasteh SC, Cho S, Ma S, Mulder T, Chen J, Pang J, Ding X, Deese A, Pellet J, Siebers N, Joas L, Salphati L, Ware J.
Xenobiotica, 2021, 51, 796–810.

Pharmacokinetics of S-epacadostat, an indoleamine 2,3-dioxygenase 1 inhibitor, in dog plasma and identification of its metabolites in vivo and in vitro.
Zhang Y, Li X, Sun Y, Liu X, Wang W, Tian J.
Biomed. Chromatogr., 2021, 35, e5226.

Simultaneous determination of loratadine and its metabolite desloratadine in beagle plasma by LC-MS/MS and application for pharmacokinetics study of loratadine tablets and omeprazole‑induced drug-drug interaction.
Zhang Y, Zhang J, Xu Q, Wang Y, Wu W, Wang W, Li X, Zhang T.
Drug Des. Devel. Ther., 2021, 15, 5109–5122.