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Canadian Methods Directory

 

DRUG NAME: CAFFEINE

Development Laboratory:

Can Test, Vancouver, British Columbia, Canada [1983]

Novamann (Ontario), Mississauga, Ontario, Canada [1994]

Novamann (Québec), Lachine, Québec, Canada [1996]

Method(s) Used:

Direct liquid extraction of caffeine and metabolites from plasma and urine for analysis using HPLC/UV and GC/MS. Solid phase extraction of caffeine and metabolites from urine for analysis using TLC.

Substances Detected:

Caffeine, theobromine, theophylline and paraxanthine. Caffeine confirmed as itself by GC/MS. Metabolites confirmed as n-butyl derivatives using GC/MS.

 

DRUG NAME: CAMPHOR

Development Laboratory:

Can Test, Vancouver, British Columbia, Canada [1983]

Method(s) Used:

Direct liquid extraction of camphor from plasma and urine for analysis using GC/FI and GC/MS. Derivatization [hydrazone] of plasma and urine extracts for HPLC/UV. Direct liquid extraction of metabolites from enzyme-hydrolysed urine for analysis using GC/MS.

Substances Detected:

Camphor, trans-isoketopinic acid, hydroxycamphor, using GC/MS.

 

DRUG NAME: CHLORPHENIRAMINE

Development Laboratory:

Can Test, Vancouver, British Columbia, Canada [1984]

Method(s) Used:

Direct liquid extraction of chlorpheniramine from plasma and enzyme-hydrolysed urine for analysis using GC/NP and GC/MS. Solid phase extraction of chorpheniramine and metabolites from urine for analysis using TLC.

Substances Detected:

Chlorpheniramine, mono-desmethylchlorpheniramine, di-desmethylchlorpheniramine, using GC/MS.

 

DRUG NAME: CLENBUTEROL

Development Laboratory:

Novamann (Ontario), Mississauga, Ontario, Canada [1984]

Can Test, Vancouver, British Columbia, Canada [1988]

Novamann (Québec), Lachine, Québec, Canada [1991]

Method(s) Used:

Direct liquid extraction of clenbuterol from plasma and urine for analysis using GC/EC and GC/MS. Solid phase extraction of clenbuterol from urine for analysis using TLC.

Direct liquid extraction of clenbuterol from plasma and urine for analysis using HPLC/UV and GC/MS. Solid phase extraction of clenbuterol from urine for analysis using TLC.

Direct liquid extraction of clenbuterol from plasma and urine for analysis using HPLC/UV and HPLC/MS/MS. Diazo derivatization of urine extracts for HPLC/UV and HPLC/MS/MS analysis.

Substances Detected:

Clenbuterol. Confirmed as itself by GC/MS and HPLC/MS/MS, and as diazo derivative by HPLC/MS/MS.

 

DRUG NAME: CROMOGLYCATE

Development Laboratory:

Novamann (Ontario), Mississauga, Ontario, Canada [1991]

Can Test, Vancouver, British Columbia, Canada [1994]

Method(s) Used:

ELISA kit developed for urine screening for cromoglycate.

Direct liquid combined with solid phase extraction of cromoglycate from plasma and urine for analysis using HPLC/MS/MS and GC/MS.

Substances Detected:

Cromoglycate, using HPLC/MS/MS and GC/MS.

 

DRUG NAME: DEMBREXINE

Development Laboratory:

Novamann (Ontario), Mississauga, Ontario, Canada [1988]

Method(s) Used:

Direct liquid extraction of dembrexine from plasma and urine for analysis using HPLC/UV and GC/MS. Solid phase extraction of dembrexine from urine for analysis using TLC.

Substances Detected:

Dembrexine, using TLC, HPLC/UV and GC/MS.

 

DRUG NAME: DEXTROMETHORPHAN

Development Laboratory:

Can Test, Vancouver, British Columbia, Canada [1983]

Method(s) Used:

Direct liquid extraction of dextromethorphan and metabolites from plasma and urine for analysis using GC/NP and GC/MS. Direct liquid extraction of dextromethorphan metabolites from enzyme-hydrolysed urine for analysis using GC/NP and GC/MS. Solid phase extraction of dextromethorphan and metabolites from urine for analysis using TLC.

Substances Detected:

Dextromethorphan in plasma and urine using GC/NP and GC/MS. Free and conjugated dextrorphan and (+)-3-methoxymorphinan in urine using TLC, GC/NP and GC/MS.

 

DRUG NAME: DIPHENHYDRAMINE

Development Laboratory:

Novamann (Ontario), Mississauga, Ontario, Canada [1984]

Method(s) Used:

Direct liquid extraction of diphenhydramine and metabolites from plasma and urine for analysis using GC/NP and GC/MS. Direct liquid extraction of diphenhydramine metabolites from enzyme-hydrolysed urine for analysis using GC/MS. Solid phase extraction of diphenhydramine from urine for analysis using TLC.

Substances Detected:

Diphenhydramine in plasma using GC/NP and GC/MS; in urine using TLC, GC/NP and GC/MS. N-desmethyl-diphenhydramine, benzhydrol and benzophenone in enzyme-hydrolysed urine using GC/MS. N-didesmethyl-diphenydramine and benzophenone in enzyme-hydrolysed and base-hydrolysed urine using GC/MS.

 

DRUG NAME: DYPHYLLINE

Development Laboratory:

Novamann (Québec), Lachine, Québec, Canada [1987]

Method(s) Used:

Direct liquid extraction of dyphylline from plasma and urine for analysis using HPLC/UV and GC/MS. Solid phase extraction of dyphylline from urine for analysis using TLC.

Substances Detected:

Dyphylline in plasma using HPLC/UV and GC/MS; in urine using TLC, HPLC/UV and GC/MS.

 

DRUG NAME: EPHEDRINE

Development Laboratory:

1. & 2. Novamann (Québec), Lachine, Québec, Canada [1984, 1996]

Method(s) Used:

1. Direct liquid extraction of ephedrine and metabolites from plasma and urine for analysis using GC/NP and GC/MS. Solid phase extraction of ephedrine and metabolites from urine for analysis using TLC.

2. Direct liquid extraction of ephedrine and metabolites from plasma and urine for analysis using HPLC/MS/MS. Solid phase extraction of ephedrine and metabolites from urirne for analysis using TLC.

Substances Detected:

Ephedrine and phenylpropanolamine in plasma and urine using GC/NP, GC/MS and HPLC/MS/MS. Ephedrine and phenylproplanolamine in urine using TLC [coincidental detection of pyrilamine which was a component of the product administered].

 

DRUG NAME: ERGONOVINE

Development Laboratory:

Novamann (Québec), Lachine, Québec, Canada [1991]

Method(s) Used:

Direct liquid extraction of ergonovine from plasma and urine for analysis using HPLC/UV, GC/MS and HPLC/MS/MS. Solid phase extraction of ergonovine from urine for analysis using TLC.

Substances Detected:

Ergononvine in plasma and urine using HPLC/UV, GC/MS and HPLC/MS/MS; in urine using TLC.

 

DRUG NAME: GLYCOPYRROLATE

Development Laboratory:

1. Novamann (Ontario), Mississauga, Ontario, Canada [1991]

2. Novamann (Québec), Lachine, Québec, Canada [1991]

Method(s) Used:

ELISA kit developed for urine screening for glycopyrrolate.

Direct liquid [ion-pair] extraction of glycopyrrolate from plasma and urine for analysis using HPLC/MS/MS and GC/MS.

Substances Detected:

Glycopyrrolate in plasma and urine using HPLC/MS/MS; in urine using GC/MS.

 

DRUG NAME: GUAIFENESIN

Development Laboratory:

Novamann (Ontario), Mississauga, Ontario, Canada [1982]

Novamann (Québec), Lachine, Québec, Canada [1987]

Novamann (Québec), Lachine, Québec, Canada [1995]

Method(s) Used:

Direct liquid extraction of guaifenesin from plasma and urine [untreated, acid-hydrolysed, enzyme-hydrolysed] for analysis using HPLC/UV and GC/MS. Solid phase extraction of guaifenesin from urine for analysis using TLC.

Direct liquid extraction of guaifenesin from plasma and enzyme-hydrolysed urine for analysis using HPLC/UV. Solid phase extraction of guaifenesin from urine for analysis using TLC.

Direct liquid extraction of guaifenesin from plasma and enzyme-hydrolyzed urine for analysis using HPLC/UV. Solid phase extraction of guaifenesin from urine for analysis using TLC.

Substances Detected:

Guaifenesin in plasma, urine, acid-hydrolysed and enzyme-hydrolysed urine using HPLC/UV and GC/MS. Guaifenesin in urine and acid-hydrolysed urine using TLC.

 

DRUG NAME: PHENYLPROPANOLAMINE

Development Laboratory:

Novamann (Québec), Lachine, Québec, Canada [1982]

Method(s) Used:

Direct liquid extraction of phenylpropanolamine from plasma and urine for analysis using GC/NP and GC/MS. Solid phase extraction of phenylpropanolamine from urine for analysis using TLC.

Substances Detected:

Phenylpropanolaminein plasma and urine using GC/NP and GC/MS; in urine using TLC. Also detected as a metabolite of ephedrine.

 

DRUG NAME: PYRILAMINE

Development Laboratory:

1. & 2. Novamann (Québec), Lachine, Québec, Canada [1983, 1996]

Method(s) Used:

Direct liquid extraction of pyrilamine and metabolites from plasma and enzyme-hydrolysed urine for analysis using GC/NP and GC/MS. Solid phase extraction of pyrilamine and metabolites from urine for analysis using TLC.

Direct liquid extraction of pyrilamine and metabolites from plasma and urine for analysis using HPLPC/MS/MS. Solid phase extraction of pyrilamine and metabolites from urine for analysis using TLC.

Substances Detected:

Pyrilamine in plasma, urine and enzyme-hydrolysed urine using GC/NP, GC/MS and HPLC/MS/MS; in urine using TLC. Two metabolites detected but not confirmed in these studies.

 

DRUG NAME: SALBUTAMOL

Development Laboratory:

Novamann (Ontario), Mississauga, Ontario, Canada [1993]

Method(s) Used:

ELISA kit developed for urine screening for salbutamol.

Substances Detected:

Kit reacted to salbutamol, clenbuterol and terbutaline.

 

DRUG NAME: TERBUTALINE

Development Laboratory:

Novamann (Québec), Lachine, Québec, Canada [1992]

Method(s) Used:

Solid phase extraction of terbutaline from plasma and urine for analysis using HPLC/MS/MS. Direct analysis of plasma and urine using ELISA kit developed by Novamann (Ontariro) for salbutamol.

Substances Detected:

Terbutaline in plasma and urine using HPLC/MS/MS.

 

DRUG NAME: THEOPHYLLINE

Development Laboratory:

Novamann (Québec), Lachine, Québec, Canada [1983]

Novamann (Ontario), Mississauga, Ontario, Canada [1988]

Novamann (Québec), Lachine, Québec, Canada [1995]

Method(s) Used:

Direct liquid extraction of theophylline from plasma and urine for analysis using HPLC/UV and GC/MS. Solid phase extraction of theophylline from urine for analysis using TLC.

Substances Detected:

Theophylline in plasma and urine using HPLC/UV and GC/MS; in urine using TLC.

 

DRUG NAME: TRIPELENNAMINE

Development Laboratory:

Can Test, Vancouver, British Columbia, Canada [1988]

Method(s) Used:

Direct liquid extraction of tripelennamine from plasma and urine for analysis using GC/NP and GC/MS. Solid phase extraction of tripelennamine from urine for analysis using TLC.

Substances Detected:

Tripelennamine in plasma and urine using GC/NP and GC/MS; in urine using TLC.