Canadian Methods Directory
DRUG NAME: CAFFEINE
Development Laboratory:
Can Test, Vancouver, British Columbia, Canada [1983]
Novamann (Ontario), Mississauga, Ontario, Canada [1994]
Novamann (Québec), Lachine, Québec, Canada [1996]
Method(s) Used:
Direct liquid extraction of caffeine and metabolites from plasma and urine for analysis using HPLC/UV and GC/MS. Solid phase extraction of caffeine and metabolites from urine for analysis using TLC.
Substances Detected:
Caffeine, theobromine, theophylline and paraxanthine. Caffeine confirmed as itself by GC/MS. Metabolites confirmed as n-butyl derivatives using GC/MS.
DRUG NAME: CAMPHOR
Development Laboratory:
Can Test, Vancouver, British Columbia, Canada [1983]
Method(s) Used:
Direct liquid extraction of camphor from plasma and urine for analysis using GC/FI and GC/MS. Derivatization [hydrazone] of plasma and urine extracts for HPLC/UV. Direct liquid extraction of metabolites from enzyme-hydrolysed urine for analysis using GC/MS.
Substances Detected:
Camphor, trans-isoketopinic acid, hydroxycamphor, using GC/MS.
DRUG NAME: CHLORPHENIRAMINE
Development Laboratory:
Can Test, Vancouver, British Columbia, Canada [1984]
Method(s) Used:
Direct liquid extraction of chlorpheniramine from plasma and enzyme-hydrolysed urine for analysis using GC/NP and GC/MS. Solid phase extraction of chorpheniramine and metabolites from urine for analysis using TLC.
Substances Detected:
Chlorpheniramine, mono-desmethylchlorpheniramine, di-desmethylchlorpheniramine, using GC/MS.
DRUG NAME: CLENBUTEROL
Development Laboratory:
Novamann (Ontario), Mississauga, Ontario, Canada [1984]
Can Test, Vancouver, British Columbia, Canada [1988]
Novamann (Québec), Lachine, Québec, Canada [1991]
Method(s) Used:
Direct liquid extraction of clenbuterol from plasma and urine for analysis using GC/EC and GC/MS. Solid phase extraction of clenbuterol from urine for analysis using TLC.
Direct liquid extraction of clenbuterol from plasma and urine for analysis using HPLC/UV and GC/MS. Solid phase extraction of clenbuterol from urine for analysis using TLC.
Direct liquid extraction of clenbuterol from plasma and urine for analysis using HPLC/UV and HPLC/MS/MS. Diazo derivatization of urine extracts for HPLC/UV and HPLC/MS/MS analysis.
Substances Detected:
Clenbuterol. Confirmed as itself by GC/MS and HPLC/MS/MS, and as diazo derivative by HPLC/MS/MS.
DRUG NAME: CROMOGLYCATE
Development Laboratory:
Novamann (Ontario), Mississauga, Ontario, Canada [1991]
Can Test, Vancouver, British Columbia, Canada [1994]
Method(s) Used:
ELISA kit developed for urine screening for cromoglycate.
Direct liquid combined with solid phase extraction of cromoglycate from plasma and urine for analysis using HPLC/MS/MS and GC/MS.
Substances Detected:
Cromoglycate, using HPLC/MS/MS and GC/MS.
DRUG NAME: DEMBREXINE
Development Laboratory:
Novamann (Ontario), Mississauga, Ontario, Canada [1988]
Method(s) Used:
Direct liquid extraction of dembrexine from plasma and urine for analysis using HPLC/UV and GC/MS. Solid phase extraction of dembrexine from urine for analysis using TLC.
Substances Detected:
Dembrexine, using TLC, HPLC/UV and GC/MS.
DRUG NAME: DEXTROMETHORPHAN
Development Laboratory:
Can Test, Vancouver, British Columbia, Canada [1983]
Method(s) Used:
Direct liquid extraction of dextromethorphan and metabolites from plasma and urine for analysis using GC/NP and GC/MS. Direct liquid extraction of dextromethorphan metabolites from enzyme-hydrolysed urine for analysis using GC/NP and GC/MS. Solid phase extraction of dextromethorphan and metabolites from urine for analysis using TLC.
Substances Detected:
Dextromethorphan in plasma and urine using GC/NP and GC/MS. Free and conjugated dextrorphan and (+)-3-methoxymorphinan in urine using TLC, GC/NP and GC/MS.
DRUG NAME: DIPHENHYDRAMINE
Development Laboratory:
Novamann (Ontario), Mississauga, Ontario, Canada [1984]
Method(s) Used:
Direct liquid extraction of diphenhydramine and metabolites from plasma and urine for analysis using GC/NP and GC/MS. Direct liquid extraction of diphenhydramine metabolites from enzyme-hydrolysed urine for analysis using GC/MS. Solid phase extraction of diphenhydramine from urine for analysis using TLC.
Substances Detected:
Diphenhydramine in plasma using GC/NP and GC/MS; in urine using TLC, GC/NP and GC/MS. N-desmethyl-diphenhydramine, benzhydrol and benzophenone in enzyme-hydrolysed urine using GC/MS. N-didesmethyl-diphenydramine and benzophenone in enzyme-hydrolysed and base-hydrolysed urine using GC/MS.
DRUG NAME: DYPHYLLINE
Development Laboratory:
Novamann (Québec), Lachine, Québec, Canada [1987]
Method(s) Used:
Direct liquid extraction of dyphylline from plasma and urine for analysis using HPLC/UV and GC/MS. Solid phase extraction of dyphylline from urine for analysis using TLC.
Substances Detected:
Dyphylline in plasma using HPLC/UV and GC/MS; in urine using TLC, HPLC/UV and GC/MS.
DRUG NAME: EPHEDRINE
Development Laboratory:
1. & 2. Novamann (Québec), Lachine, Québec, Canada [1984, 1996]
Method(s) Used:
1. Direct liquid extraction of ephedrine and metabolites from plasma and urine for analysis using GC/NP and GC/MS. Solid phase extraction of ephedrine and metabolites from urine for analysis using TLC.
2. Direct liquid extraction of ephedrine and metabolites from plasma and urine for analysis using HPLC/MS/MS. Solid phase extraction of ephedrine and metabolites from urirne for analysis using TLC.
Substances Detected:
Ephedrine and phenylpropanolamine in plasma and urine using GC/NP, GC/MS and HPLC/MS/MS. Ephedrine and phenylproplanolamine in urine using TLC [coincidental detection of pyrilamine which was a component of the product administered].
DRUG NAME: ERGONOVINE
Development Laboratory:
Novamann (Québec), Lachine, Québec, Canada [1991]
Method(s) Used:
Direct liquid extraction of ergonovine from plasma and urine for analysis using HPLC/UV, GC/MS and HPLC/MS/MS. Solid phase extraction of ergonovine from urine for analysis using TLC.
Substances Detected:
Ergononvine in plasma and urine using HPLC/UV, GC/MS and HPLC/MS/MS; in urine using TLC.
DRUG NAME: GLYCOPYRROLATE
Development Laboratory:
1. Novamann (Ontario), Mississauga, Ontario, Canada [1991]
2. Novamann (Québec), Lachine, Québec, Canada [1991]
Method(s) Used:
ELISA kit developed for urine screening for glycopyrrolate.
Direct liquid [ion-pair] extraction of glycopyrrolate from plasma and urine for analysis using HPLC/MS/MS and GC/MS.
Substances Detected:
Glycopyrrolate in plasma and urine using HPLC/MS/MS; in urine using GC/MS.
DRUG NAME: GUAIFENESIN
Development Laboratory:
Novamann (Ontario), Mississauga, Ontario, Canada [1982]
Novamann (Québec), Lachine, Québec, Canada [1987]
Novamann (Québec), Lachine, Québec, Canada [1995]
Method(s) Used:
Direct liquid extraction of guaifenesin from plasma and urine [untreated, acid-hydrolysed, enzyme-hydrolysed] for analysis using HPLC/UV and GC/MS. Solid phase extraction of guaifenesin from urine for analysis using TLC.
Direct liquid extraction of guaifenesin from plasma and enzyme-hydrolysed urine for analysis using HPLC/UV. Solid phase extraction of guaifenesin from urine for analysis using TLC.
Direct liquid extraction of guaifenesin from plasma and enzyme-hydrolyzed urine for analysis using HPLC/UV. Solid phase extraction of guaifenesin from urine for analysis using TLC.
Substances Detected:
Guaifenesin in plasma, urine, acid-hydrolysed and enzyme-hydrolysed urine using HPLC/UV and GC/MS. Guaifenesin in urine and acid-hydrolysed urine using TLC.
DRUG NAME: PHENYLPROPANOLAMINE
Development Laboratory:
Novamann (Québec), Lachine, Québec, Canada [1982]
Method(s) Used:
Direct liquid extraction of phenylpropanolamine from plasma and urine for analysis using GC/NP and GC/MS. Solid phase extraction of phenylpropanolamine from urine for analysis using TLC.
Substances Detected:
Phenylpropanolaminein plasma and urine using GC/NP and GC/MS; in urine using TLC. Also detected as a metabolite of ephedrine.
DRUG NAME: PYRILAMINE
Development Laboratory:
1. & 2. Novamann (Québec), Lachine, Québec, Canada [1983, 1996]
Method(s) Used:
Direct liquid extraction of pyrilamine and metabolites from plasma and enzyme-hydrolysed urine for analysis using GC/NP and GC/MS. Solid phase extraction of pyrilamine and metabolites from urine for analysis using TLC.
Direct liquid extraction of pyrilamine and metabolites from plasma and urine for analysis using HPLPC/MS/MS. Solid phase extraction of pyrilamine and metabolites from urine for analysis using TLC.
Substances Detected:
Pyrilamine in plasma, urine and enzyme-hydrolysed urine using GC/NP, GC/MS and HPLC/MS/MS; in urine using TLC. Two metabolites detected but not confirmed in these studies.
DRUG NAME: SALBUTAMOL
Development Laboratory:
Novamann (Ontario), Mississauga, Ontario, Canada [1993]
Method(s) Used:
ELISA kit developed for urine screening for salbutamol.
Substances Detected:
Kit reacted to salbutamol, clenbuterol and terbutaline.
DRUG NAME: TERBUTALINE
Development Laboratory:
Novamann (Québec), Lachine, Québec, Canada [1992]
Method(s) Used:
Solid phase extraction of terbutaline from plasma and urine for analysis using HPLC/MS/MS. Direct analysis of plasma and urine using ELISA kit developed by Novamann (Ontariro) for salbutamol.
Substances Detected:
Terbutaline in plasma and urine using HPLC/MS/MS.
DRUG NAME: THEOPHYLLINE
Development Laboratory:
Novamann (Québec), Lachine, Québec, Canada [1983]
Novamann (Ontario), Mississauga, Ontario, Canada [1988]
Novamann (Québec), Lachine, Québec, Canada [1995]
Method(s) Used:
Direct liquid extraction of theophylline from plasma and urine for analysis using HPLC/UV and GC/MS. Solid phase extraction of theophylline from urine for analysis using TLC.
Substances Detected:
Theophylline in plasma and urine using HPLC/UV and GC/MS; in urine using TLC.
DRUG NAME: TRIPELENNAMINE
Development Laboratory:
Can Test, Vancouver, British Columbia, Canada [1988]
Method(s) Used:
Direct liquid extraction of tripelennamine from plasma and urine for analysis using GC/NP and GC/MS. Solid phase extraction of tripelennamine from urine for analysis using TLC.
Substances Detected:
Tripelennamine in plasma and urine using GC/NP and GC/MS; in urine using TLC.